Beksich  DABS, 4um
(200 x 4.6 mm)
流動(dòng)相:
A: 25 mM醋酸鈉 pH=6,6 /乙腈 (80/20), B: 乙腈/異丙醇 (40/60)
梯度:
0min. 0% B, 4min. 5%B, 8min. 20%B, 15min. 25%B, 27min. 60%B, 28min. 100%B, 32 min. 100%B
流速: 1 ml/min.
檢測(cè)波長(zhǎng): VIS 436 nm

溫度: 40℃

Beksich FMOC-H, 5um(250 x 4 mm)
流動(dòng)相:A:50 mM醋酸鈉pH 4.2/乙腈(80/20)   B=50mM醋酸鈉pH 4.2 /乙腈 (20/80)
梯度:0min. 0B, 35min. 40%B,45min. 70%B, 50min.100%B, 55 min. 100%B.

檢測(cè)器: Fluor.:  Ex.: 263 nm，  Em.: 310 nm
流速: 1 ml/min.   進(jìn)樣量: 10ul   溫度: 40℃

Column: Beksich OPA,
3 um (150 x 4.6mm)

Analysis of Amino Acids by pre-column Derivatisation with通過(guò) OPA/3-MPA衍生化分析氨基酸
梯度:
A: 25mM 磷酸鈉緩沖鹽 (pH=7,2)/THF (995/5)
B: 25 mM 磷酸鈉緩沖鹽(pH=7,2) 甲醇/乙腈 (50/35/15)
梯度: 0-3min. 0%B, bei 20min. 35%B, 25min.50%B, 36min. 60%B, 40min. 70%B, 43-45min.
100%B.

 流速:1ml/min. Fluor.-檢測(cè)器: Ex330nm EM. 450nm